Immunomodulatory Treatment of Infertility and Subfertility in Men with Elevated Antisperm Antibodies

Etiologycaly, possible causes of subfertility or infertility in men are poorly defined, but one important category may be elevated level of antisperm antibodies (ASA) caused by infective and/or auto-immune diseases of male reproductive system. In this study, blood sera from 45 infertile men of different age with ASA positive ELISA test were examined for the serum level of ASA before and after treatments with 1,25-dihydroxy-Vitamin-D3 and Dexamethasone. We observed 23 infertile men treated with VitaminD3/Dexamethasone during 30 days, 12 infertile men treated with Dexamethasone only during 30 days and 10 infertile men without any treatment. All selected patients showed poor parameters of semen analysis and high level of ASA serum concentration (>75 U/ml). Serum concentration of ASA in nontreated group (312.6 U/ml), Dexamethasone only treated group (288.0) and Vitamin-D3/Dexamethasone treated group (123.6 U/ml) are significantly different. In addition, serum level of ASA in VitaminD3+Dexamethasone treated group was significant less as compared to the level before the treatment (P<0.01). Immunomodulatory and suppressive effects of Dexamethasone and Vitamin D3 regarding antibody production, co-stimulatory molecules expression, immune cells communications and profile of cytokine network could explain these results. Since the approach is relatively new, this is the first study of Vitamin D3 and Dexamethasone treatment concerning the suppression of ASA production.